Analytical and Bio-Analytical Techniques in Metabolomics

Metabolomics is the relative analysis of endogenous metabolites and to identify and quantify metabolites from a biological sample. Metabolites represent various groups of low molecular weight structures including lipids, amino acids, peptides, nucleic acids, organic acids, vitamins, thiols and carbohydrates, which makes global analysis a tiresome challenge. The rapid development of analytical platforms, including GC, HPLC, UPLC, CE coupled to MS and NMR spectroscopy, could enable separation, detection, characterization and quantification of such metabolites and related metabolic pathways. To detect all metabolites in a biological sample, the complexity of the metabolome and the miscellaneous properties of metabolites has no single analytical platform can be applied. By single analytical technique, the integrated use of modern instrumental analytical approaches is beneficial to increase detected metabolites that cannot be achieved. These analytical platforms have been frequently used to provide tactful and valid detection of metabolites in a biofluid sample. Continuous development of these analytical platforms will accelerate widespread use and incorporation of metabolomics into systems biology.

  • Translational biomarker discovery
  • Nuclear magnetic resonance (NMR)
  • Mass spectrometry
  • Ratio analysis of NMR/MS
  • Analytical variability and issues
  • Chromatographic methods in metabolomics
  • GC/MS, LC-MS, CE/MS based metabolomics
  • MALDI mass spectrometric imaging
  • Metabolite extraction
  • Metabolomics screening
  • NMR fingerprinting, profiling

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